In situ outer dynein arm from Chlamydomonas reinhardtii in a pre-power stroke state
Resolution: 38.0 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8bwy
Q-score: 0.039
Zimmermann N, Noga A, Obbineni JM, Ishikawa T
EMBO J (2023) 42 pp. e112466-e112466 [ DOI: doi:10.15252/embj.2022112466 Pubmed: 37051721 ]
- Dynein light chain roadblock-type 2 protein (18 kDa, Protein from Chlamydomonas reinhardtii)
- Thioredoxin (12 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain (12 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain (10 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain 2a (15 kDa, Protein from Chlamydomonas reinhardtii)
- In situ outer dynein arm (Cellular component from Chlamydomonas reinhardtii)
- Dynein intermediate chain 2 (77 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Chlamydomonas reinhardtii)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynein light chain (10 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain (12 kDa, Protein from Chlamydomonas reinhardtii)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Chlamydomonas reinhardtii)
- Docking complex 1/2 protein (11 kDa, Protein from Chlamydomonas reinhardtii)
- Outer arm dynein beta heavy chain (530 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain (13 kDa, Protein from Chlamydomonas reinhardtii)
- Calmodulin (35 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain (10 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Chlamydomonas reinhardtii)
In situ outer dynein arm from Chlamydomonas reinhardtii in the post-power stroke state
Resolution: 30.3 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8bx8
Q-score: 0.043
Zimmermann N, Noga A, Obbineni JM, Ishikawa T
EMBO J (2023) 42 pp. e112466-e112466 [ DOI: doi:10.15252/embj.2022112466 Pubmed: 37051721 ]
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (11 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 1 (22 kDa, Protein from Tetrahymena thermophila)
- Thioredoxin (14 kDa, Protein from Tetrahymena thermophila)
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Tetrahymena thermophila)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila)
- Outer arm dynein beta heavy chain (530 kDa, Protein from Tetrahymena thermophila)
- In situ outer dynein arm (Cellular component from Chlamydomonas reinhardtii)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain roadblock-type 2 protein (18 kDa, Protein from Tetrahymena thermophila)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 4a (17 kDa, Protein from Tetrahymena thermophila)
- Dynein intermediate chain 2 (76 kDa, Protein from Tetrahymena thermophila)
In situ outer dynein arm from Chlamydomonas reinhardtii in an intermediate state
Zimmermann N, Noga A, Obbineni JM, Ishikawa T
EMBO J (2023) 42 pp. e112466-e112466 [ DOI: doi:10.15252/embj.2022112466 Pubmed: 37051721 ]
In situ outer dynein arm from Chlamydomonas reinhardtii in an intermediate
Zimmermann N, Noga A, Obbineni JM, Ishikawa T
EMBO J (2023) 42 pp. e112466-e112466 [ DOI: doi:10.15252/embj.2022112466 Pubmed: 37051721 ]
Cilia from MOT7 deletion mutant of Chlamydomonas
Kutomi O, Yamamoto R, Hirose K, Mizuno K, Nakagiri Y, Imai H, Noga A, Obbineni JM, Zimmermann N, Nakajima M, Shibata D, Shibata M, Shiba K, Kita M, Kigoshi H, Tanaka Y, Yamasaki Y, Asahina Y, Song C, Nomura M, Nomura M, Nakajima A, Nakachi M, Yamada L, Nakazawa S, Sawada H, Murata K, Mitsuoka K, Ishikawa T, Wakabayashi KI, Kon T, Inaba K
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf3621 Pubmed: 33637535 ]
Chlamydomonas cilia with MOT7-BCCP labeled
Kutomi O, Yamamoto R, Hirose K, Mizuno K, Nakagiri Y, Imai H, Noga A, Obbineni JM, Zimmermann N, Nakajima M, Shibata D, Shibata M, Shiba K, Kita M, Kigoshi H, Tanaka Y, Yamasaki Y, Asahina Y, Song C, Nomura M, Nomura M, Nakajima A, Nakachi M, Yamada L, Nakazawa S, Sawada H, Murata K, Mitsuoka K, Ishikawa T, Wakabayashi KI, Kon T, Inaba K
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf3621 Pubmed: 33637535 ]
Microtubule doublet structure from WT Chlamydomonas as a control for MOT7 mutants
Kutomi O, Yamamoto R, Hirose K, Mizuno K, Nakagiri Y, Imai H, Noga A, Obbineni JM, Zimmermann N, Nakajima M, Shibata D, Shibata M, Shiba K, Kita M, Kigoshi H, Tanaka Y, Yamasaki Y, Asahina Y, Song C, Nomura M, Nomura M, Nakajima A, Nakachi M, Yamada L, Nakazawa S, Sawada H, Murata K, Mitsuoka K, Ishikawa T, Wakabayashi KI, Kon T, Inaba K
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf3621 Pubmed: 33637535 ]